Experiment records

Printable Western blot experiment and troubleshooting worksheets

Use the experiment record before and during a run. If a result fails, use the troubleshooting record to document the pattern, checks already completed and the next planned change. Fields stay in this browser unless you export them.

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Worksheet 1

Western blot experiment record

Record planned and actual conditions, including antibody lots, transfer results and the unsaturated image used for analysis.

Project / experiment IDDateOperatorTarget protein / modificationExpected apparent molecular weightSample source, treatment and collection timePositive biological controlNegative / knockout controlLoading or total-protein normalization planLysis buffer, inhibitors and clarificationProtein concentration method and resultAmount and final volume per laneSample buffer, reducer and heating conditionGel system, percentage, voltage and run timeMembrane, pore size, transfer system and settingsTransfer stain and observationBlocker, concentration, buffer and durationPrimary antibody catalog, lot, dilution, buffer and incubationSecondary antibody, dilution and incubationWash buffer, volume, times and agitationSubstrate / channels and imager settingsExposure series and unsaturated image selectedRaw-file and uncropped-image locationObservations and deviations from plan

Worksheet 2

Western blot troubleshooting record

Record the observed pattern, checks already completed, competing explanations and the one variable you plan to change next. Keep the remaining conditions as consistent as possible.

Case / experiment IDDateObserved whole-blot patternUncropped image and exposure recordMarker and expected-band positionPositive, negative and normalization controlsTransfer stain resultGlobal, target-, lane- or position-specific?First experimental step showing a problemLeading explanationAlternative explanationCheck that would distinguish the twoOne variable to change nextConditions that must remain fixedCondition before changeCondition after changeResult after re-runCurrent conclusion and unresolved questions