Western blot calculators for loading, dilutions and buffer preparation

Protein loading volume

With concentration in µg/µL and target amount in µg, the result is directly in µL.

This arithmetic does not prove the loading amount is inside the detection system’s linear range.

Sample mix and batch volumes

Calculate sample, loading buffer, optional reducer and water per lane, then scale for lane count and excess.

Use this batch calculation only for samples with the same concentration; calculate sample and water separately when concentrations differ.

Prepare a 1:N antibody dilution with intermediate-dilution guidance

When the direct volume is below the reliable pipetting range, the calculator suggests a workable intermediate dilution.

Prepare the intermediate dilution in a compatible diluent and account for adsorption and storage stability.

C₁V₁ = C₂V₂

Stock and final concentrations must use the same unit; final volume is entered in mL.

Tween-20 volume for TBST

Calculate from final volume and target v/v percentage. A common 0.1% is only a starting point.

Gel-range and transfer-risk helper

Use target and normalization sizes to identify the gel range to verify, whether a gradient gel is worth comparing, and small-protein retention risk.

This helper deliberately avoids a universal gel percentage; verify the exact manufacturer separation chart.